REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal Anti-β-Tubulin III antibody produced in mouse	Sigma-Aldrich	Cat. #T8660; AB_477590
Anti-MAP2 (2a+2b) antibody, Mouse monoclonal	Sigma-Aldrich	Cat. #M1406; AB_477171
Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody, Mouse monoclonal	Sigma-Aldrich	Cat. #C9205; AB_476889
Bacterial and virus strains
AAV9.Syn.GCaMP6s.WPRE.SV40	Addgene	Cat. # 100843-AAV9
Experimental models: Organisms/strains
Sprague-Dawley rat	University of Barcelona	N/A
Chemicals, peptides, and recombinant proteins
DAPI-fluoromount–G	Shouthern Biotech	Cat. # 0100–20
L-15 Medium	Thermo Fisher	Cat. # 11415–049
MEM Eagle’s Minimum Essential Medium	Thermo Fisher	Cat. # 21090–022
Glutamax	Fisher Scientific	Cat. # 35050–038
Glucose	Sigma-Aldrich	Cat. #G5400-1KG
Horse Serum	Thermo Fisher	Cat. # 26050088
Fetal bovine serum	Thermo Fisher	Cat. # 16140071
FUDR	Sigma-Aldrich	Cat. #F0503
B27	Fisher Scientific	Cat. # 17504044
Poly-L-Lysine	Sigma-Aldrich	Cat. #P4707
Boric Acid	Sigma-Aldrich	Cat. #B19334-500G
Borax Anhydrous	Sigma-Aldrich	Cat. # 71997–100G
PFA	Sigma-Aldrich	Cat. #P6148
PBS	Thermo-Fisher	Cat. # 10010023
Triton	Sigma	Cat. # T8787
Normal Donkey Serum	Jackson Immuno research	Cat. # 017-000-121
Deposited data
Neuronal cultures on PDMS topographical patterns: experiments and simulations (https://doi.org/10.17632/8yp4xb6d3s.1)	Mendeley Data	https://data.mendeley.com/datasets/8yp4xb6d3s/1
Software and algorithms
NETCAL: An interactive platform for large-scale, NETwork and population dynamics analysis of CALcium imaging recordings	Zenodo34,68	https://zenodo.org/record/1119026#.Y20nUXbMKUk
Gephi	Bastian et al., 200969	https://gephi.org/
MATLAB 2018a	MathWorks	https://www.mathworks.com/
Origin 9.1	OriginLab	https://www.originlab.com/
Other
PDMS kit: Sylgard 184	Ellsworth Adhesives	Cat. # 2137054