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Immunofluorescence detection the HIF-1α expression of hippocampus tissue CA1, CA3 and DG area (A). The average nuclear HIF-1α fluorescence density of hippocampus in three fields of view per slice was analyzed in each group by the ImageJ software (B–D). Compared with other three groups, sepsis up-regulated the HIF-1a expression in the hippocampus of mice.**:p < 0.01, ***:p < 0.001,****:p < 0.0001, ns:p > 0.05.
