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. 2022 Dec 7;13:1025931. doi: 10.3389/fimmu.2022.1025931

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Copyright © 2022 Weng, Zhang, Ma, Zhou, Jia, Wan, Cui, Ruan, Shao, Wu, Wang, Zhang and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of antibodies in the serum of immunized mice. Levels of antibody detected by ELISA at 2 and 4 weeks after immunization. Samples were diluted 1/1000 in PBS buffer. (A) Schedule for DNA vaccine immunization in mice. (B-D) Anti A39, B31, B21 protein IgG antibody; (E-G) Anti A39, B31, B21 protein IgG1 antibody; (H-J) Anti A39, B31, B21 protein IgG2b antibody. Data are shown as mean±SD, n = 5. Statistical analyses performed by two-way ANOVA (ns, No significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).