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. 2022 Dec 7;13:1025931. doi: 10.3389/fimmu.2022.1025931

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Copyright © 2022 Weng, Zhang, Ma, Zhou, Jia, Wan, Cui, Ruan, Shao, Wu, Wang, Zhang and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of cytokine production. Two weeks after immunization, mouse splenocytes were stimulated in vitro with fusion proteins A39, B31, B21 (5 µg/mL), P/I or PBS for 16 h. Antigen-specific IFN-γ secreting cells were measured by ELISPOT; cytokines in the supernatant were detected using ELISA. (A) Display of IFN-γ specific spot forming cells. Three representative samples from each group were selected for the showcase. (B) IFN-γ responses between groups were compared. (C) Specific release of TNF-α, IL-2, and IL-1β between groups. Data are shown as mean±SD, n = 5. Statistical analyses performed by one-way ANOVA (ns, No significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).