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. 2022 Dec 7;13:1038347. doi: 10.3389/fimmu.2022.1038347

Figure 4.

Figure 4

T-HIFU and M-HIFU tumor debris characterization. Mice bearing EG7 tumors were treated with T-HIFU or M-HIFU ablation and immediately after treatment tumor debris was extracted. Tumor debris was characterized for RNA (A), DNA (B) and protein content (C). Each lane represents tumor debris and matched control sample obtained from an individual mouse. Western blot analysis (D) and quantification (E) of TGF-β, IL-10 and actin in tumor debris and corresponding surrounding control tissue. Each lane represents tumor debris and matched control sample obtained from an individual mouse. Statistical significance was calculated using a repeated measures two-way ANOVA followed by Bonferroni’s multiple comparison tests (T-HIFU n = 2, M-HIFU n = 7). IL-10: Interleukin-10; M-HIFU: mechanical-high intensity focused ultrasound; T-HIFU: thermal-high intensity focused ultrasound; TGF-β: transforming growth factor-beta. p < 0.01 **, p < 0.001 ***.