TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| A. actinomycetemcomitans strains | ||
| CU1000N | Spontaneous nalidixic acid-resistant mutant of rough clinical isolate CU1000 | 23 |
| CU1060 | Spontaneous smooth, nonadherent variant of CU1000 | 23 |
| CU1060N | Spontaneous nalidixic acid-resistant mutant of CU1060 | 23 |
| JP2 | High-level leukotoxin producer; nonadherent, smooth | 50 |
| Y4 | Nonadherent, smooth | 39 |
| AA1360 | Derivative of CU1000N; tadA2::IS903φkan; Kmr | 23 |
| AA1332 | Derivative of CU1000N; tadB7::IS903φkan; Kmr | 23 |
| AA1359 | Derivative of CU1000N; tadC3::IS903φkan; Kmr | 23 |
| AA1577 | Derivative of CU1000N; tadD19::IS903φkan; Kmr | 23 |
| Plasmids | ||
| pEK2 | pJAK16 with tadA from CU1000N expressed from tac promoter | 23 |
| pJAK16 | Broad-host-range mobilizable IncQ vector derived from pMMB67; Cmr, tac promoter, lacIq | 56 |
| pRK21761 | oriT mutant derivative of RK2 used to mobilize plasmids from E. coli to A. actinomycetemcomitans; Kmr AprtetA::lacZYA | 45 |
| pSK69 | pJAK16 with tadCD from CU1000N expressed from tac promoter | 23 |
| pSK98 | pJAK16 with tadC from CU1000N expressed from tac promoter | 23 |
| pSK128 | pJAK16 with tadB from CU1000N expressed from tac promoter | 23 |