Figure 2.

Separation of HSA and mass spectrometry analysis

A) Separation of human serum albumin (ALB) samples in SDS-PAGE gel is shown. The arrow and annotation “ALB” indicate the migration position of HSA. The annotation of samples is indicated at the top of the gel. Migration of molecular mass markers is indicated as “markers”. B) Two mass spectra show representative spectra of albumin recovered after exposure to MDA-MB-468 cells and water solubilized HSA. Representative mass spectra of all 5 types of albumin samples are shown in Supplementary Figure S1. The intensity of peaks and m/z values are indicated. Inserts show examples of peaks with assigned PTMs. See Table 1 for annotation of all PTMs.