Fig. 1. PhaseScan workflow.

a Droplets are generated using a flow-focussing microfluidic device controlled by automated syringe pumps and then imaged in wells by fluorescence microscopy. b At the droplet generating junction, aqueous solutions are combined under laminar flow before droplet formation. c Brightfield microscopy image of droplet generation (left) and combined fluorescence images of droplet generation (right) showing fluorescence of EGFP (green) and Alexa647 (magenta) barcodes for FUS^G156E and PEG, respectively. d, e Epifluorescence microscopy images of trapped microdroplets, with EGFP and Alexa647 fluorescence corresponding to FUS^G156E and PEG concentration, respectively. f Classification of droplets as phase separated (red outline) or homogeneous (blue outline) according to distribution of EGFP fluorescence. g Phase separated (left) and homogeneous (right) microdroplets imaged according to EGFP (top) and Alexa647 fluorescence (middle) and subsequent phase separation classification (bottom). Images correspond to the highlighted regions in (d–f). h Liquid condensates merge over time in microdroplets. i Phase diagram of EGFP-FUS^G156E vs. PEG 6000 concentration, 50 mM Tris pH 7.4, 150 mM KCl. Red and blue data points in the scatter plot correspond to individual microdroplets classified as phase separated or homogeneous, respectively. The heat map corresponds to the probability of phase separation as determined by an SVM classifier trained on the droplet scatter plot. N = 2754 droplets. Yellow and cyan crosses correspond to phase separated and homogeneous behaviour as determined by manual pipetting experiment. Source data are provided as a Source Data file. Parts of this figure are reproduced with permission from Geiger et al.¹².