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. 2022 Dec 21;13:7845. doi: 10.1038/s41467-022-35265-7

Fig. 4. Generation of multidimensional phase diagrams using the PhaseScan platform.

Fig. 4

a, b 3D phase diagram of EGFP-tagged FUSG156E vs. PEG 6000 vs. 1,6-HD concentration. N = 3904 droplets. c Epifluorescence microscopy images of trapped microdroplets with EGFP (green), Alexa546 (yellow), and Alexa647 (red) fluorescence corresponding to FUSG156E and 1,6-HD, and PEG concentrations, respectively. d–f 2D slices of the 3D phase diagram, with outline colours corresponding to the red, green and blue planes shown in (b), respectively. Red and blue data points in scatter plots correspond to individual microdroplets classified as phase separated or homogeneous, respectively. The heat map corresponds to the probability of phase separation as determined by an SVM classifier trained on the droplet scatter plot. Source data are provided as a Source Data file.