Extended Data Fig. 6. Longitudinal retrospective analysis of epitope persistence, somatic signatures and ctDNA data.

a) Venn diagrams of patients with longitudinal screening, pre and post infusion biopsies where available showing protein altering mutation overlap and targeted neoantigen persistence patterns. b) Somatic signature analysis of somatic exome mutations and their correlation with known somatic signatures in the COSMIC database. Signature 13 has previously been associated with APOBEC activity. c) Bespoke ctDNA assay for patient 0506 at day −5 and day 0 timepoints showing truncal mutations in gray and targeted neoantigens in aqua and blue respectively. The PREP neoantigen (blue) is detectable by ctDNA and is at lower ctDNA concentrations than predicted truncal mutations.