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. 2022 Nov 10;615(7953):687–696. doi: 10.1038/s41586-022-05531-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a) Intracellular cytokine staining upon activation with cognate peptide-HLA. NeoTCR T cells produce a polyfunctional cytokine profile on antigen encounter. Cells from the clinical final cell product were stimulated overnight with plate-bound peptide-HLA. Percent of CD8 cells positive for the given markers is shown. b and c) T cells were stained with Viafluor membrane bound dye, stimulated with plate-bound peptide-HLA overnight, and proliferation measured 4 days later. b) Concentration-dependent proliferation of individual patient products. CD3/CD28 stimulation positive control indicated by [+] and mis-match compact negative control (used at 1000 ng/mL) indicated by [−]. Y-axis inverted; increased proliferation has lower ViaFluor MFI signal due to dilution of the dye after cell division. c) Leftward shift in the Viafluor MFI indicates increasing proliferation (left). Mis-matched peptide-HLA served as a negative control, and CD3/CD28 stimulation as a positive control (right).