TABLE 1.
Bacterial strains and plasmids and relevant properties
| Strain or plasmid | Genotype or relevant phenotype | Source or reference |
|---|---|---|
| Strainsa | ||
| SM10λpir | thi-1 thr-1 leuB6 supE44 tonA21 lacY1 recA::RP4-2-Tc::Mu | |
| JSG210 | ATCC 14028s (WT) | ATCC |
| JSG430 | pho24 pmrA::Tn10d (PhoPc PmrA−) | 9 |
| JSG437 | phoP::Tn10d-tet pmrA505 zjd::Tn10d-cam (PhoP− PmrAc) | 9 |
| JSG435 | ATCC 14028s pmrA 505 zjd::Tn10d-cam (PmrAc) | 9 |
| JSG421 | pmrA::Tn10d (PmrA−) | 9 |
| JSG485 | JSG435 pmrF1::Tn10d | 8 |
| JM109 | F′traD36 lacI Δ(lacZ)M15 proA+B+/e14− (mcrA) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17 (rK− mK−) relA1 supE44 recA1 | Promega |
| JSG224 | phoN2 zxx::6251 Tn10d-cam (CS019) | 15 |
| JSG844 | JSG435 rpsL (PmrAc Smr) | This study |
| JSG1097 | JSG844; ΔpmrH | This study |
| JSG911 | JSG844; ΔpmrF | This study |
| JSG868 | JSG844; ΔpmrI | This study |
| JSG1098 | JSG844; ΔpmrJ | This study |
| JSG883 | JSG844; ΔpmrK | This study |
| JSG884 | JSG844; ΔpmrL | This study |
| JSG1046 | JSG421 pmrM::luc | This study |
| JSG1047 | JSG435 pmrM::luc | This study |
| JSG1048 | JSG485 pmrM::luc | This study |
| JSG989 | JSG224 pmrD::luc | This study |
| Plasmids | ||
| pGPL01 | Firefly luciferase recorder, suicide vector | 9 |
| pKAS32 | rpsL suicide vector | 19 |
| pSR01 | pKAS32; ΔpmrH | This study |
| pSR02 | pKAS32; ΔpmrI | This study |
| pSR03 | pKAS32; ΔpmrK | This study |
| pSR04 | pKAS32; ΔpmrL | This study |
| pSR05 | pKAS32; ΔpmrF | This study |
| pSR06 | pKAS32; ΔpmrJ | This study |
| pLB02 | Firefly luciferase recorder, suicide vector | 7 |
| pGPLM02 | pGPL01 with pmrM EcoRI-KpnI PCR fragment amplified with JG252 and JG253 | This study |
| pMRD1 | pGPL01 with pmrD EcoRI-KpnI PCR fragment amplified with JG177 and JG178 | This study |
| pLB2223 | 118-bp pmrHFIJKLM operon promoter-fragment (JG122 and JG123) in pLB02 (EcoRI-KpnI) | This study |
| pQPA01 | pQE30 containing the pmrA (WT) gene amplified with primers JG106 and JG107 (BamHI-PstI) | This study |
| pQP505 | pQE30 containing the pmrA-constitutive (JSG435) gene amplified with primers JG106 and JG107 (BamHI-PstI) | This study |
| pQE30 | Amino-terminal His tag vector | Qiagen |
a
Strain SMI0λpir is E. coli; the other strains are S. enterica serovar Typhimurium.