TABLE 2.

Primers used for construction of each nonpolar deletion

Deleted gene	Primer	Primer sequence (5′ to 3′)
pmrH	JG234	CAT GCC ATG GCA TTG AAA GCC GCT TTT C
	JG235	GCT CTA GAC CCG TGC TGT CTG ACA GG
	JG236	CAT GCC ATG GGA TAG CAG GAC AAT AAG CAT G
	JG237	CGG AAT TCG TGA TGC ACC GGG ATC TC
pmrF	JG183	CAT GCC ATG GGT CAA ACA TGC TTA TTG TCC TGC TAT C
	JG181	GCT CTA GAC CCT TGA CCG TAA TCC CCG TG
	JG184	CAT GCC ATG GGC CGT TTA CTG AGG AAA GCC ACC AAT G
	JG202	CGG AAT TCG CCG TTA TAG CTG AAC GCG CC
pmrI	JG185	CAT GCC ATG GTG GTG GCT TTC CTC AGT AAA C
	JG187	GCT CTA GAC AGG ACA GCC TGT TTC GCA AAT CG
	JG186	CAT GCC ATG GCG ATA TCG CGG AAC GCG CAT C
	JG188	CGG AAT TCC CAG GTA TTG AAC GAC TGC GE
pmrJ	JG245	CAT GCC ATG GGC GTA AAC CGA CTT TCG TCA TGA TGC G
	JG246	GCT CTA GAC AGC GGT AAA CGT CGC ATT CG
	JG247	CAT GCC ATG GGG CAG CGA GCG CCT CAT GAT G
	JG248	CGG AAT TCG AAA GAA CAT AGG TGG GCA GTT TC
pmrK	JG191	CAT GCC ATG GGC GTA TCG ATT TCA TCA TGA G
	JG193	GCT CTA GAC ACC ATG CGT GGC AGA CTC AC
	JG192	CAT GCC ATG GGG TGT TAA TTC AGT ATC GGC C
	JG196	CGG AAT TCG CAA GCC ATC ATG ATC TGG CG
pmrL	JG194	CAT GCC ATG GCA GAA CGA CGC CGA TCA TTT AGG C
	JG193	GCT CTA GAC ACC ATG CGT GGC AGA CTC AC
	JG195	CAT GCC ATG GGG CAT AAT GGG CGT AAT GTG G
	JG196	CGG AAT TCG CAA GCC ATC ATG ATC TGG CG