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. 2022 Dec 18;12(1):2148560. doi: 10.1080/22221751.2022.2148560

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — In vivo evaluation of different gene-deleted African swine fever viruses (ASFVs). (A) Timeline for animal experiments. The groups of commercial pigs were inoculated twice with 10^5.00 TCID₅₀ of different gene-deleted ASFVs and then challenged at 28 dpi with lethal ASFV-GZ virus inoculated intramuscularly (i.m.). Pigs were observed for 21 days post-challenge and then euthanized. (B) The rectal temperature of the pigs inoculated with different gene-deleted ASFVs and challenged with lethal ASFV-GZ virus. *P < 0.05, the mean rectal temperature of pigs in the ASFV-GZΔI177L group was significantly higher than that in the ASFV-GZΔI177LΔCD2vΔMGF group at 10–23 days post-inoculation and 13–16 days post-challenge. (C) The clinical score of the pigs were recorded and calculated after inoculation and challenge. **P < 0.01, the mean clinical score in the ASFV-GZΔI177L group was significantly higher than that in the ASFV-GZΔI177LΔCD2vΔMGF group. (D) Survival curve of the pigs after inoculation and challenge.