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Paraformaldehyde fixation of AC inhibits lymphocyte proliferation in response to C. neoformans. C. neoformans (A) or SEB (B) was used to stimulate T cells in the presence of irradiated AC or AC that had been fixed with 1% paraformaldehyde for 5 min. [3H]TdR incorporation was determined during the last 18 h of culture. ∗, P < 0.05 compared to unstimulated cultures. NS, not significantly different compared to stimulated cells. The experiment was repeated twice with similar results.
