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An NH4Cl-sensitive pathway is used to process live and heat-killed C. neoformans. PBMC were cultured alone or stimulated with autoclaved, heat-killed (56°C for 30 min), or live C. neoformans in the presence or absence of NH4Cl (1 mM). ∗, P < 0.05 compared to stimulated PBMC. The experiment was repeated three times with similar results.
