Table.
List of Antibodies, Fluorochromes, Clones, Usages, and Manufacturers
| Antibody | Fluorochrome | Clone | Usage | Manufacturer |
|---|---|---|---|---|
| Rat anti-mouse CD45 | PE/Cy7 | 30-F11 | FCM | BioLegend |
| Rat anti-mouse F4/80 | FITC | BM8 | FCM | BioLegend |
| Rat anti-mouse CD11b | APC/Cy7 | M1/70 | FCM | BioLegend |
| Hamster anti-mouse CD11c | PE | N418 | FCM | BioLegend |
| Rat anti-mouse I-A/I-E (MHC II) | Pacific Blue | M5/114.15.2 | FCM | BioLegend |
| Rat anti-mouse CD3 | PE/Cy7 | 17A2 | FCM | BioLegend |
| Rat anti-mouse CD4 | APC | RM4-5 | FCM | eBioscience |
| Rat anti-mouse CD8 | APC/Cy7 | YTS156.7.7 | FCM | BioLegend |
| Rat anti-mouse CD16/CD32 | N/A | 2.4G2 | Fc block | BD Bioscience |
| Rat anti-mouse F4/80 | N/A | N/A | IF | Thermo Fisher Scientific |
| Hamster anti-mouse CD11c | Alexa Fluor 647 | N418 | IF | BioLegend |
| Rat anti-mouse Ly6G | Alexa Fluor 647 | 1A8 | IF | BioLegend |
| Rat anti-mouse I-A/I-E (MHC II) | Alexa Fluor 488 | M5/114.15.2 | IF | BioLegend |
| Rabbit anti-mouse CD3 | N/A | N/A | IF | Abcam |
| Rat anti-mouse CD4 | N/A | GK1.5 | IF | Thermo Fisher Scientific |
| Rat anti-mouse CD8 | N/A | 5H10-1 | IF | BioLegend |
| Rat anti-mouse CD31 | FITC | 390 | IF | BD Bioscience |
| Rabbit anti-mouse LYVE-1 | N/A | N/A | IF | AngioBio |
| Goat anti-rabbit IgG (H+L) | Alexa Fluor 488 | N/A | IF | Thermo Fisher Scientific |
| Goat anti-rabbit IgG (H+L) | Cy3 | N/A | IF | Jackson ImmunoResearch |
| Goat anti-rat IgG (H+L) | Alexa Fluor 555 | N/A | IF | Thermo Fisher Scientific |
APC, allophycocyanin; CD, cluster of differentiation; Cy, cyanine; FCM, flow cytometry; FITC, fluorescein isothiocyanate; IF, immunofluorescence; LYVE-1, lymphatic vessel endothelial hyaluronan receptor 1; N/A, not applicable; PE, phycoerythrin.