UL41 deficiency facilitated IFN-β induction and attenuated DEV replication in ducks. (A to C) Ducks were infected intramuscularly with 100 TCID50 of DEV-WT or DEV-dUL41, and IFN-β (A), Mx (B), and OASL (C) production was measured by qPCR at the indicated time points. (D) Ducks were infected intramuscularly with 100 TCID50 of DEV-WT or DEV-dUL41, and virus genome copy numbers in the spleen were monitored by qPCR at the indicated time points. All samples from the mock control and DEV-infected groups were examined in triplicate. *, P < 0.05; **, P < 0.01; ns, no significant difference.