FIG 3.

CVA9 expansion dependency on faf-BSA and palmitate concentrations. (A) Fractionation of untreated (gray), 0.01% faf-BSA-treated (red) or 0.0005% faf-BSA-treated (orange) CVA9 after separation in a 5%-to-20% sucrose gradient using ultracentrifugation. The peak for intact virions corresponded to fractions 15 to 17, for expanded virions to fractions 12 to 14, and for empty virions to fractions 7 to 10. (B) Analysis of CVA9 treated with 0.005% faf-BSA in the presence of an excess of palmitate (pink) after ultracentrifugation in a 5%-to-20% sucrose gradient. For comparison, 0.005% faf-BSA-treated CVA9 was ultracentrifuged the same way without addition of palmitate and is shown in purple. Similar analysis of CVA9 treated with endosomal ion concentrations with (blue) or without (light blue) addition of palmitate is also shown. The peak for intact virions corresponded to fractions 15 to 18, for expanded virions to fractions 12 to 14, and for empty capsids to fractions 7 to 10. Buffer composition is shown in Table 1.