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. Author manuscript; available in PMC: 2022 Dec 21.
Published in final edited form as: Bioconjug Chem. 2021 Oct 7;32(10):2233–2244. doi: 10.1021/acs.bioconjchem.1c00403

Figure 10:

Figure 10:

Comparison of non-templated NCL and CAN reaction rates using L32 peptides (12a, 13a, 15, and 18). Relative peak area of ligated product is shown at various time points (relative to TCEP addition). CAN ligations were fit to first-order rate constants. For non-templated NCL, peak areas of both product (14a) and L32-N reactant (12a^) were calculated using estimated extinction coefficients, as described in the supplementary methods. Each data point represents the average of two independent experiments, with error bars showing standard deviation.