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. Author manuscript; available in PMC: 2022 Dec 21.
Published in final edited form as: Bioconjug Chem. 2021 Oct 7;32(10):2233–2244. doi: 10.1021/acs.bioconjchem.1c00403

Figure 8:

Figure 8:

One-pot SPAAC, CAN, desulfurization, and linker cleavage assembly strategy for L32-far (dotted line) and L32-close (solid line), showing location of Ddap attachment points at specified Lys residues.