TABLE 2.
Treatment | No. of PMNC (P)
|
No. of phagocytizing PMNC (P)
|
Fluorescence intensity (P)
|
|||
---|---|---|---|---|---|---|
2 days | 7 days | 2 days | 7 days | 2 days | 7 days | |
RB6-8C5 | 7.2 ± 1.4 (<0.02) | 239.4 ± 74 (<0.009) | 0.4 ± 0.1 (<0.02) | 58.8 ± 9 (<0.009) | 563 ± 23 (<0.014) | 218 ± 10 (NS)b |
Rat IgG | 81 ± 16.5 | 64.4 ± 5.5 | 22.8 ± 4.4 | 18.4 ± 2.9 | 273 ± 16.8 | 224 ± 13 |
Phagocytic activity of granulocytes in heparinized whole blood of noninfected mice 2 days after a single inoculation and 7 days after repeated inoculations with either MAb RB6-8C5 (n = 10) or rat IgG (n = 10). The number of leukocytes was evaluated with a hemacytometer, and flow cytometry was used to calculate the proportion of polymorphonuclear cells (PMNC). The values are given as 107 PMNC/liter ± standard error of the mean.
NS, not significant.