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. 2000 Nov;68(11):6162–6167. doi: 10.1128/iai.68.11.6162-6167.2000

TABLE 2.

Quantification of phagocytic activity of granulocytesa

Treatment No. of PMNC (P)
No. of phagocytizing PMNC (P)
Fluorescence intensity (P)
2 days 7 days 2 days 7 days 2 days 7 days
RB6-8C5 7.2 ± 1.4 (<0.02) 239.4 ± 74 (<0.009) 0.4 ± 0.1 (<0.02) 58.8 ± 9 (<0.009) 563 ± 23 (<0.014) 218 ± 10 (NS)b
Rat IgG 81 ± 16.5 64.4 ± 5.5 22.8 ± 4.4 18.4 ± 2.9 273 ± 16.8 224 ± 13
a

Phagocytic activity of granulocytes in heparinized whole blood of noninfected mice 2 days after a single inoculation and 7 days after repeated inoculations with either MAb RB6-8C5 (n = 10) or rat IgG (n = 10). The number of leukocytes was evaluated with a hemacytometer, and flow cytometry was used to calculate the proportion of polymorphonuclear cells (PMNC). The values are given as 107 PMNC/liter ± standard error of the mean. 

b

NS, not significant.