FIG 4.

Effects of ATFK and AKU-002 on C4,C9-FA lipid labeling. (A) Compound structures. (B) Concentration dependence of inhibition of C4,C9-FA incorporation into DAG, TAG, PC and PE. BTC-normalized fluorescence values are expressed as a fraction of the no-inhibitor control. Data points are means from three independent experiments with standard deviation represented with bars. (C) Live-cell images of parasites labeled with C4,C9-FA in the presence of DMSO vehicle, 10 μM ATFK, or 10 μM AKU-002. DNA was stained with Hoechst 33342 and is pseudocolored red. Scale bar, 3 μm.