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Immunofluorescent staining shows GFP localization of mitochondrially targeted C. trachomatis proteins after removal of the MTS. HeLa cells on coverslips were transfected with GFP-tagged C. trachomatis proteins with the MTS removed (CT### no MTS) and incubated for 24 h before staining with 100 nM MitoTracker (red). Bar = 10 μm. Colocalization scatterplots were determined for each representative image, and Pearson coefficients are reported.
