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. 2022 Nov 29;10(6):e03511-22. doi: 10.1128/spectrum.03511-22

FIG 3.

FIG 3

Function of LfsT in regulating vital phage genes. (A) LacZ activity in the indicated double transformants carrying pDN19lacΩ::Pgp71 (Pgp71-lacZ) evaluated by the corresponding β-galactosidase activities. pUCP18, empty vector; pCT72N, pUCP18::gp72; pCT71N, pUCP18::gp71; pCTX, pUCP18::lfsT. (B) LacZ activity in the indicated E. coli DH5α double transformants carrying pDN19lacΩ::Pgp75 (Pgp75-lacZ) or pDN19lacΩ::Pgp71 (Pgp71-lacZ). pET32a(+) is an empty expression vector. pOEX, pET32a(+)::lfsT. The overexpression of lfsT was started by the addition of 1.5 mM isopropyl β-d-1-thiogalactopyranoside (IPTG). (C) LacZ activity of different phage gene promoter-lacZ fusions in P8D carrying pUCP18 or pCT72N. (D) LacZ activity corresponding to the results in panel C for P8W carrying pUCP18 or pCT72N. All of the double transformants were grown in LB medium supplemented with 100 mg/L ampicillin, streptomycin, and tetracycline. The experiments were independently replicated three times, and each sample was tested in triplicate. Data were analyzed by ANOVA with Tukey’s multiple-comparison test (α < 0.05) to examine the mean differences between the data groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars show standard deviations.