FIG 4.

Negative effects of LfsT on lysogenic phage development. (A and B) RT-qPCR assay for gp58 (encoding a YqaJ viral recombinase family protein) and gp36 (encoding a phage integrase) in different strains (colored lines) infected with phage PP9W2 at the indicated time intervals. (C and D) Lysogeny frequencies of phage PP9W2 in the indicated strains after 2 h (C) or 12 h (D) of growth. The experiments were independently replicated three times, and each sample was tested in triplicate. Data were analyzed by one-way ANOVA with Tukey’s multiple-comparison test (α < 0.05) to examine the mean differences between the indicated groups. **, P < 0.01; ***, P < 0.001. Error bars show standard deviations.