FIG 6.

Role of LfsT in fatty acid metabolism. (A) Schematic of the faoA (encoding the fatty acid oxidation complex α-subunit) and PA2550 (encoding a probable acyl-CoA dehydrogenase) genes engaging in the partial dehydrogenation-oxidation of fatty acids. (B) HDT activity assays of the indicated strains. (C) Growth curves of the related strains using 0.1% (wt/vol) palmitic acid as the sole carbon and energy source (supplemented with 100 mg/L ampicillin) for 12 h. (D and E) RT-qPCR assay of faoA (encoding the fatty acid oxidation complex α-subunit) and PA2550 (encoding a probable acyl-CoA dehydrogenase) in the indicated strains at mid-log phase (8 h in LB medium supplemented with 100 mg/L ampicillin). Each sample was tested in triplicate (C to E) or sextuplicate (B), and the experiments were independently replicated three times. Data were analyzed by one-way ANOVA with Tukey’s multiple-comparison test (α < 0.05) to compare the mean differences between the data groups. Error bars show standard deviations. ***, P < 0.001.