TABLE 1.
Bacterial strains, phages, and plasmids used in this work
| Strain, phage, or plasmid | Genotype and/or description | GenBank accession no., reference, or source |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| P8W | Wild-type P. aeruginosa clinical isolate | NZ_CP081477.2 |
| P8D | lfsT deletion derivative of P8W | This work |
| P8D/pCTX | Complemented (lfsT) strain of P8D | This work |
| PR1 | Tn5G insertion mutant (wapR) of P8W | This work |
| PR2 | Tn5G insertion mutant (wapP) of P8W | This work |
| PR3 | Tn5G insertion mutant (waaC) of P8W | This work |
| PR4 | Tn5G insertion mutant (waaC) of P8W | This work |
| PR5 | Tn5G insertion mutant (lfsT) of P8W | This work |
| P8D/pUCP18 | P8D carrying pUCP18 | This work |
| P8W/pUCP18 | P8W carrying pUCP18 | This work |
| P8D/pCT72N | P8D carrying pUCP18::gp72 | This work |
| P8D/pCT71N | P8D carrying pUCP18::gp71 | This work |
| P8W/pCT72N | P8W carrying pUCP18::gp72 | This work |
| P8W/pCT71N | P8W carrying pUCP18::gp71 | This work |
| E. coli | ||
| DH5α | F− ϕ80 lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 endA1 hsdR17(rK− mK+) supE44 λ− thi-1 gyrA96 relA1 phoA | TianGen |
| S17-1 | RP4-2 Tc::Mu Km::Tn7 Tpr Smr Pro Res− Mod+ | TransGen |
| JM109 | endA1 recA1 gyrA96 thi hsdR17(rK− mK+) relA1 supE44D(lac-proAB) [F′ traD36 proAB laqIqZΔM15] | Biomed |
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm(DE3) | Biomed |
| DH5α/pET32a(+) | DH5α carrying pET32a(+) | This work |
| DH5α/pOEX | DH5α carrying pET32a(+)::lfsT | This work |
| Phages | ||
| PP9W | Excised phage isolated previously | This work |
| PP9W2 | Lyses P8W or is lysogenic in it | OM141125 |
| PP27 | Excised phage isolated previously | This work |
| Plasmids | ||
| pRK2013Tn5G | Tn5G-carrying plasmid; Kmr Gmr | 69 |
| pEX18Tc | Gene replacement vector; Tcr oriT+ sacB+ | Weihui Wu |
| pEX18Tc::UD | lfsT gene knockout vector | This work |
| pUCP18 | Broad-host-range shuttle vector; Apr | Weihui Wu |
| pCTX | lfsT gene complementation vector | This work |
| pCT72N | pUCP18::gp72 | This work |
| pCT71N | pUCP18::gp71 | This work |
| pMD19 (Simple) | Linearized T vector for cloning promoter fragments; Apr | TaKaRa |
| pCP75 | gp75 gene promoter cloned into pMD19 | This work |
| pCP72 | gp72 gene promoter cloned into pMD19 | This work |
| pCP71 | gp71 gene promoter cloned into pMD19 | This work |
| pCP13 | gp13 gene promoter cloned into pMD19 | This work |
| pCP09 | gp09 gene promoter cloned into pMD19 | This work |
| pCP04 | gp04 gene promoter cloned in pMD19 | This work |
| pCP01 | gp01 gene promoter cloned into pMD19 | This work |
| pCPP | potA gene promoter cloned into pMD19 | This work |
| pCPF | faoA gene promoter cloned into pMD19 | This work |
| pCPN | popN gene promoter cloned into pMD19 | This work |
| pCP2 | PA2550 gene promoter cloned into pMD19 | This work |
| pDN19lacΩ | Promoterless lacZ fusion vector; Spr Smr Tcr | Weihui Wu |
| pDN75 | gp75 gene promoter cloned into pDN19lacΩ | This work |
| pDN71 | gp71 gene promoter cloned into pDN19lacΩ | This work |
| pDN13 | gp13 gene promoter cloned into pDN19lacΩ | This work |
| pDN09 | gp09 gene promoter cloned into pDN19lacΩ | This work |
| pDN04 | gp04 gene promoter cloned into pDN19lacΩ | This work |
| pDN01 | gp01 gene promoter cloned into pDN19lacΩ | This work |
| pDNP | potA gene promoter cloned into pDN19lacΩ | This work |
| pDNF | faoA gene promoter cloned into pDN19lacΩ | This work |
| pDNN | popN gene promoter cloned into pDN19lacΩ | This work |
| pDN2 | PA2550 gene promoter cloned into pDN19lacΩ | This work |
| pET32a(+) | Fusion vector for an N-terminal His tag; Apr | Novagen |
| pOEX | his-lfsT fusion in the pET32a(+) vector; Apr | This work |
| pEASY-T1 | Cloning vector for plotting the standard curves; Apr Kanr | TransGen |
| pEASY-L | Partially integrated fragment in pEASY-T1 | This work |
| pEASY-1789 | Partial PA1789 fragment in pEASY-T1 | This work |