TABLE 2.

Mean number of DRMs per study participant identified by Sanger sequencing and NGS at different detection thresholds^a

ARV class	NGS detection threshold (%)	Mean no. of DRMs by:
		Agreement (Sanger and NGS)	Discrepancy
			NGS alone	Sanger alone
Any DRM (NRTI and/or NNRTI)	1	4.65	0.67	0.07
	2	4.65	0.54	0.07
	5	4.65	0.38	0.07
	10	4.65	0.21	0.07
	15	4.63	0.09	0.09
	20	4.55	0.05	0.17
NRTI DRMs	1	2.65	0.24	0.06
	2	2.65	0.20	0.06
	5	2.65	0.16	0.06
	10	2.65	0.08	0.06
	15	2.64	0.04	0.08
	20	2.59	0.02	0.12
NNRTI DRMs	1	2.09	0.43	0.01
	2	2.09	0.33	0.01
	5	2.09	0.22	0.01
	10	2.09	0.13	0.01
	15	2.08	0.05	0.02

^aARV, antiretroviral; DRMs, drug resistant mutations; NGS, next generation sequencing; NRTI, nucleoside reverse transcriptase inhibitors; NNRTI, nonnucleoside reverse transcriptase inhibitors.