TABLE 2.
Leptospira strains isolated over a 5-year period (2002 to 2007) from humans and peri-domiciliary rats in Iquitos, Perua
| Source (# Isolates) |
Species | CAAT/pFGE | Tm/oC | Alleleb | Consistency (%) | Dates MM/YY |
Age/yrs | Agreement |
|---|---|---|---|---|---|---|---|---|
| Human (28) | interrogans | svBataviae | 80.2 | AG4 | 1/1 | 01/07 | 11 | 1/1 |
| svCanicola | 80.6 | AG5 | 4/4 | 03/03 to 07/04 | 14 to 15 | 4/6 | ||
| svCopenhageni | 80.2 | AG6 | 9/9 | 03/03 to 03/07 | 11 to 15 | 9/9 | ||
| noguchii | Australis | 80.4 | AA1 | 1/1 | 04/04 | 14 | 1/1 | |
| Bataviae | 80.4, 80.6, 80.8, 81.0 | AA1, AA2, AA3, AA4 | 1/1, 3/3, 2/2 | 07/03 to 06/07 | 11 to 15 | 6/6 | ||
| santarosai | Pyrogenes | 85.0 | GCA6 | 1/1 | 12/04 | 14 | 1/1 | |
| Tarassovi | 84.8 | GCA7 | 4/4 | 05/04 to 04/07 | 11 to 14 | 4/4 | ||
| Rat (6) | interrogans | svCopenhageni | 80.2 | AG4 | 3/3 | 12/02 to 02/03 | 15 to 16 | 3/6 |
a
CFX96-derived Tm and relevant serotyping info are indicated (minimum of three replicates).
b
AG alleles amplify with 11108f_AGA forward primer but not *_AAA forward, whereas AA alleles amplify with *_AAA forward but not *_AGA.