TABLE 2.
The primer information
| Primer name | Sequences (5′–3′)a | References |
|---|---|---|
| P42-F | CGGAATTCCTTGTTAAAAAAATCAAAGAAGAA | This study |
| P42-R | GCGTCGACTTAATCCTGCTTGATTTCAG | |
| P97-F | CGGAATTCACTACAAAAGAAGGTAAAAGAGAA | This study |
| P97-R | GCGTCGACTTATTTAGATTTTAATTCCTGATT | |
| pYA3493-F | AACGCTGGTGAAAGTAAAAGATG | This study |
| pYA3493-R | CAGACCGCTTCTGCGTTCT | |
| pET-28a-F | TAATACGACTCACTATAGGG | This study |
| pET-28a-R | GCTAGTTATTGCTCAGCGG | |
| IFN-γ-F | TCAAGTGGCATAGATGTGGAAGAA | 60 |
| IFN-γ-R | TGGCTCTGCAGGATTTTCATG | |
| IL-4-F | ACAGGAGAAGGGACGCCAT | 60 |
| IL-4-R | GAAGCCCTACAGACGAGCTCA | |
| β-actin-F | CCCTAAGGCCAACCGTGAA | 60 |
| β-actin-R | CAGCCTGGATGGCTACGTACA |
a
Underlined nucleotides denote enzyme restriction sites.