TABLE 2.
qPCR assays in this studya
| Group | Gene | Primers | Annealing temp (°C) | Standard | Reference |
|---|---|---|---|---|---|
| Total bacteria | 16S rRNA | Uni331 F, Uni797 R | 60 | E. coli JM109 | 50 |
| Methanogens | mcrA | qmcrA-F, qmcrA-R-d | 60 | M. smithii DSM 861 | 51 |
| SRB | dsrA | DSR1-F, DSR-R | 60 | D. piger DSM 749 | 52 |
| Acetogens | acsB | ACS_f, ACS_r | 52 | B. hydrogenotrophica DSM 10507 | 53 |
SRB, sulfate-reducing bacteria; mcrA, subunit of methyl coenzyme M reductase genes; dsrA, subunit of dissimilatory (bi)sulfite reductase genes; acsB, subunit of acetyl-CoA synthase genes.