The relationship of Ralstonia Pickettii and unsaturated fatty acids. (A) The biological processes that R. Pickettii participated in. (B) Comparative genomic analysis of the fatty acid metabolizing enzymes between R. Pickettii and other species. Gray dot indicates that the taxon has the coding gene of the corresponding enzyme. Ward map of fatty acid metabolizing enzymes R. pickettii specifically encoded, phospholipase A1 (EC 3.1.1.32), phospholipase A2 (EC 3.1.1.4), medium-chain acyl-CoA dehydrogenase (EC 1.3.8.7), and long-chain acyl-CoA dehydrogenase (EC 1.3.8.8). (C) The relative abundance of R. pickettii based on qRT-PCR in the matched cohort. (D) The concentration of sodium oleate and sodium linoleate after cocultured R. pickettii (ATCC27511) with sodium oleate (300 μM) and sodium linoleate (800 μM) for 48 h (n = 3). Wilcoxon rank-sum test was used to determine the significance. ****, P < 0.0001.