TABLE 1.
Red fluorescence protein (RFP) promoter reporter plasmids and primers used in this study (30)
| Plasmid name | Backbone plasmid (digested with) | Inserted promoter (PCR-amplified DNA fragments digested with NotI and BamHI) |
Inserted reporter (PCR product digested with BamHI and EcoRI) |
||||
|---|---|---|---|---|---|---|---|
| Upstream of | Size (bp) | Primer name | Primer sequence | Primer name | Primer sequence | ||
| P-bfrB-RFP | pMV262 (NotI–EcoRI) | BCG_3904 | 200 | P-bfrB-F(NotI) | gcggccgcTAACGACACGCTGCCGAATAT | mCh-F(BamHI) | ccgggatccATGGTGAGCAAGGGCGAGG |
| P-bfrB-R(BamHI) | ccgggatccAAAATGCAGATTAACGCAGGTAA | mCh-R(EcoRI) | ccggaattcCTACTTGTACAGCTCGTCCAT | ||||
| P-mbtB-RFP | pMV262 (NotI–EcoRI) | BCG_2397c | 242 | P-mbtB-F(NotI) | gcggccgcATCGGAGAGCACGGTGTCCAGG | mCh-F(BamHI) | ccgggatccATGGTGAGCAAGGGCGAGG |
| P-mbtB-R(BamHI) | ccgggatccAAACCCTCCCCTGTTAGCACAG | mCh-R(EcoRI) | ccggaattcCTACTTGTACAGCTCGTCCAT | ||||
| P-ideR-RFP | pMV262 (NotI–EcoRI) | BCG_2724 | 213 | P-ideR-F(NotI) | gcggccgcGTTCGTCAGATCGAGCGCGACG | mCh-F(BamHI) | ccgggatccATGGTGAGCAAGGGCGAGG |
| P-ideR-R(BamHI) | ccgggatccTCAGCACCCTCCATTGCAGCTGA | mCh-R(EcoRI) | ccggaattcCTACTTGTACAGCTCGTCCAT | ||||