Comparison of IncHI2 plasmids harboring mcr-9 obtained in this study and a plasmid from a nonhuman source. The plasmid name, length in kilobases, species, and isolation information (source, location, and year) are shown above each sequence. Plasmids from this study are labeled in black, and pSTN0717-64-1 (GenBank accession number AP022511.1) is labeled in blue. §, plasmid sequences that were completed by hybrid assembly. The mcr genes are indicated by a red asterisk (*), and the mcr genetic context arrays are shown next to the asterisk. Heavy metal resistance operons and resistance regions are labeled in orange and red, respectively. Gray shading connects highly similar sequences. cov, coverage; ID, identity.