TABLE 3.
Metabolites identified in cell-free extracts from different fungal strains
| Compound | Retention times (min) for UV/MS spectra | m/z |
M. anisopliae strain: |
||
|---|---|---|---|---|---|
| ΔFPPS1 strain(cps)a | WT (cps) | MaUV-HV (cps) | |||
| Subglutinols | |||||
| C | 4.54/4.75 | 443.2792 | 2.17 × 107 | No detection | 1.97 × 107 |
| Analog 45 | 8.31/8.38 | 365.2839 | No detection | 3.77 × 106 | No detection |
| Destruxins | |||||
| A | 4.57/4.69 | 578.36542 | 7.09 × 109 | 7.33 × 108 | 8.03 × 109 |
| B | 5.46/5.66 | 594.39687 | 6.38 × 109 | 5.29 × 108 | 5.93 × 109 |
| D | 4.23/4.36 | 624.37177 | 3.20 × 109 | 2.99 × 108 | 3.29 × 109 |
| A2 | 4.23/4.33 | 564.35016 | 2.35 × 109 | 1.34 × 108 | 2.01 × 109 |
| B2 and dihydro-Ab | 5.12/5.18 | 580.38168 | 6.91 × 109 | 1.45 × 108 | 7.85 × 109 |
| Unidentified | 7.32/7.39 | 509.29932 | 2.24 × 109 | 2.03 × 107 | 8.07 × 108 |
cps means the peak high of MS.
Destruxins B2 and dihydro-A are enantiomers that are not distinguished by our detection methods.