FIG 3.
Maternal PMB does not impact the TB10.4-specific CD4 T cell response in offspring. (A) Experimental schema. Pregnant BALB/c dams were treated with polymyxin B in drinking water (1 mg/mL) starting at gestational day (GD) 17 until delivery, when antibiotic treatment was stopped. Pups were immunized with 106 CFU of BCG subcutaneously at postnatal day 10, and antigen specific immunity was assessed 4 weeks postvaccination. (B) Splenic cell counts in BCG-immunized mice. (C and D) Absolute counts of CD4 and CD8 T cells in the spleen. (E) Representative flow plot of TB10.4-specific CD4 T cells. (F) TB10.4-specific CD4 T cells in spleens. (G) PPD-specific IgG in pup sera. (H) Representative flow plot of CD4 T cells producing TNF-α or IL-2. (I and J) Numbers of TNF-α- or IL-2-positive CD4 T cells. Shown as mean ± SD. Data show two or three independent experiments. n = 9 to 15 per group. *, P < 0.05.