FIG 2.

Bioinformatics analysis of Sar1. (A) Amino acid alignment of Sar1 proteins from N. bombycis and other microsporidian species. (B) Phylogenetic tree of Sar1 proteins from N. bombycis and other microsporidian species. Protein sequences were aligned using ClustalW. The maximum likelihood (ML) tree was constructed using MEGA 6.0 software, and bootstrap analyses were performed by employing Jones-Taylor-Thornton (JTT) model-based distance matrices generated from 1,000 resamplings of the alignments. Values at the branches indicate bootstrap support.