. 2022 Oct 27;10(6):e00719-22. doi: 10.1128/spectrum.00719-22

TABLE 1.

Primer sequences for PCR

Gene	Primer direction	Primer sequence (5′–3′)
NbSar1	Forward	CCGGAATTCATGGAAGAAGCCAAAAAA
NbSar1	Reverse	TTGCGGCCGCTTATTTCTTTTGTTGGTTTAT

NbSec23-1	Forward	CCGGAATTCATGGCTTACTATCCAAATTTTATG
NbSec23-1	Reverse	CCGCTCGAGTTATTCATCACTACTCACAACAA

NbSec23-2	Forward	GGAGGCCAGTGAATTCATGGAAGAAGCAATCAGAGAAATCG
NbSec23-2	Reverse	TCATCTGCAGCTCGAGCTGCAGGTCATATTCCTCATAAG

NbSec24-1	Forward	CCGGAATTCATGACAGACGATTCTTCTCAAGTTTA
NbSec24-1	Reverse	TTGCGGCCGCCTACCACCACACTTCCCTTACATTA

NbSec24-2	Forward	CATGGAGGCCGAATTCATGTTTAAGATTGTCCTTAAAAGT
NbSec24-2	Reverse	GCTAGTTATGCGGCCGCTTATGTTGATGATCCTCGTACAAA

NbSec13	Forward	CCGGAATTCATGGAGTCCCATAAAATCCAC
NbSec13	Reverse	CCGCTCGAGTTATTCACCACATTTACTAAG

NbSec31-1	Forward	CGCCATATGATGATAAGTCTTTATGAACCCG
NbSec31-1	Reverse	TTGCGGCCGCTTAATAAACTAATTGAACCAGTGT

NbSec31-2	Forward	CGCCATATGATGAAAATCAACAAAAGGTG
NbSec31-2	Reverse	TTGCGGCCGCTTAATAAACTAATTGGACCAGTG