TABLE 2.
Primer sequences for RNAi and qPCR
| Gene (purpose of primer) | Primer direction | Primer sequence (5′–3′) |
|---|---|---|
| NbSar1 (RNAi) | Sense | CCAAGUCCCAAAUUGAAAUTT |
| Antisense | AUUUCAAUUUGGGACUUGGTT | |
| Negative control (RNAi) | Sense | UUCUCCGAACGUGUCACGUTT |
| Antisense | ACGUGACACGUUCGGAGAATT | |
| NbSar1 (qPCR) | Forward | AATGCGGGTAAGACTACTCTGG |
| Reverse | CAAGCAACTCGAGCTGCTTTGTG | |
| N. bombycis β-tubulin (qPCR) | Forward | TTCCCTTCCCTAGACTTCACTTC |
| Reverse | CAGCAGCCACAGTCAAATACC | |
| NbSec23-1 (qPCR) | Forward | GCGAAAGAAATCCAACTCCTC |
| Reverse | CGTAATCACTGTCCCACTAGAA | |
| NbSec24-1 (qPCR) | Forward | GACAGACGATTCTTCTCAAGTTTATTC |
| Reverse | TCTTCATTATAATTCTCGGGTTGGA | |
| NbSec24-2 (qPCR) | Forward | CCTTTCCTAATTTCTATCCTCCTCAT |
| Reverse | CCTTATCATTCTCTGCCCTTTCT | |
| NbSec13 (qPCR) | Forward | GTGGAGGTGTGTCCTGATAATG |
| Reverse | AGAGAGACTATAGACGGGTTCAC | |
| NbSec31-1 (qPCR) | Forward | TCCACGAGTAGTTCCGAGTATG |
| Reverse | CGGGTACAGAATCCACTTGATG | |
| NbSec31-2 (qPCR) | Forward | CACGTGTAGTTCCGAGTATGAG |
| Reverse | TACAGGAGGCCTAGTACAGAAT | |
| BmGAPDH (qPCR) | Forward | TTCATGCCACAACTGCTACA |
| Reverse | AGTCAGCTTGCCATTAAGAG | |