TABLE 4.
Quality features claimed by the manufacturer for the RT-PCR kits assayed
| RT-PCR kita | Target gene(s) | LODb (no. of copies/mL) | Sensitivity (%) | Specificity (%) |
|---|---|---|---|---|
| RIDA | E | 4.3 | 100 | 100 |
| Viasure | ORF1ab, N | 20 | 95 | 100 |
| Vircell | N, E | 50 | 100 | 100 |
| LightMix | N, RdRP, and E | 10 | 100 | 100 |
| 1copy | E, RdRP | 200 | 100 | 100 |
| Logix Smart | RdRP | 600 | 100 | 100 |
a
RIDA gene kit (R-Biopharm AG, Darmstadt, Germany; catalog no. PG6815RUO); Viasure kit (CerTest Biotec, Zaragoza, Spain; catalog no. VS-NCO212L); Vircell SARS-CoV-2 RT-PCR kit (Granada, Spain; catalog no. RTPCR001); LightMix modular assay (Roche, Berlin, Germany; catalog no. 06754155001); 1copy qPCR multikit (1drop, Gyeonggi, South Korea; catalog no. M22MD100M), and Logix Smart kit (Co-Diagnostics, Salt Lake City, UT, USA; catalog no. COVID-K-001).
b
LOD, limit of detection.