Table 2.
Drug induced growth inhibition of ZR75 colonies in agar assay.
| Set | No. of data set | Drug effect (a) days-1, (mean (std. dev.)) | Growth inhibition (%) |
|---|---|---|---|
| Control | 6 | 0 | 0 |
| H5 | 7 | 0.0081 (0.0026) | 33.75 (5.4) |
| H10 | 7 | 0.0109 (0.0054) | 45.42 (11.2) |
| H25 | 7 | 0.0055 (0.0032) | 22.92 (6.6) |
| H50 | 7 | 0.0053 (0.0071) | 22.09 (14.7) |
| P1 | 6 | 0.0120 (0.0058) | 50 (12.0) |
| P5 | 6 | 0.0129 (0.0062) | 53.75 (12.9) |
| P10 | 5 | 0.0236 (0.0019) | 98.34 (3.9) |
| P20 | 3 | 0.0535 (0.0214) | 100* |
| H5P10 | 4 | 0.0200 (0.0023) | 83.34 (4.7) |
| H10P5 | 6 | 0.0224 (0.0030) | 93.34 (6.2) |
| H10P10 | 6 | 0.0224 (0.0055) | 93.34 (11.4) |
| H25P5 | 5 | 0.0225 (0.0046) | 93.75 (9.5) |
| H25P10 | 5 | 0.0225 (0.0056) | 93.75 (11.6) |
| H25P20 | 4 | 0.0315 (0.0073) | 100* |
| H50P20 | 4 | 0.0482 (0.0183) | 100* |
The drug effect parameter a is estimated using model (4) by fixing k=1e6 µm2, A0 = 200 µm2, and the growth rate of the control is set as r=0.0240 (0.0042). The overall growth rate of treated colonies is rtreat =r-a and growth inhibition is calculated as % GI=(1-(rtreat/r)) ×100. * Note that while calculating GI value for P20, H25P20, H50P20, as value of r<a, r-a becomes negative resulting in %GI>100, which is rounded off to 100%.