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. 2022 Oct 11;10(6):e02469-22. doi: 10.1128/spectrum.02469-22

FIG 3.

FIG 3

Adhesion of various staphylococci to corneocytes. Various staphylococcal species and strains were tested for adhesion to healthy human corneocytes: S. epidermidis Δica (AH2983), S. epidermidis Δica Δaap (AH2984), MRSA USA300 (AH3669), MRSA MW2 WT (AH4477), MRSA MW2 ΔmgrA (AH4478), S. hominis (AH5037), and S. carnosus (AH5687). (A) The percent area of adhesion in 10 images from three independent experiments (n = 30) was measured with Fiji ImageJ and analyzed in GraphPad Prism. Statistical significance was analyzed using the Kruskal-Wallis nonparametric test. Statistical significance of all groups except for MRSA MW2 ΔmgrA are mapped to S. epidermidis Δica (***, P = 0.0005; ****, P < 0.0001). Statistical significance of MRSA MW2 ΔmgrA is mapped to MRSA MW2 WT (***, P = 0.0001). (B) Representative green-channel, red-channel, and red- and green-channel overlay images of each species and strain tested in the corneocyte adhesion assay.