FIG 5.
SasG expression promotes S. carnosus adhesion to corneocytes. (A) pALC2073 empty vector (EV), full-length SasG-expressing strains AH5908 (pALC2073-sasGCOL) and AH5905 (pALC2073-sasGMW2), and AH6012 (pALC2073-sasGMW2ΔA) were tested for the ability to adhere to healthy human corneocytes. The percent area of adhesion in 10 images from three independent experiments (n = 30) was measured with Fiji ImageJ and analyzed in GraphPad Prism. Statistical significance was analyzed using the Kruskal-Wallis nonparametric test. Statistical significance of all groups except for pALC2073-sasGMW2ΔA is mapped to pALC2073 EV (P < 0.0001). Statistical significance of pALC2073-sasGMW2ΔA is mapped to pALC2073-sasGMW2 (P < 0.0001). (B) Representative green-channel, red-channel, and red- and green-channel overlay images of each species and strain tested in the corneocyte adhesion assay.