TABLE 3.
Comparison of kinetic properties of glucose-6-phosphate dehydrogenases
| Organism | G6P with fixed conc. of NADP+ |
G6P with fixed conc. of NAD+ |
NADP+ with fixed conc. of G6P |
NAD+ with fixed conc. of G6P |
kcat/Km(NADP+)/ kcat/Km(NAD+) |
Quarternary structure | Subunit mol. wt. (kDa) | pH optima | References | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Km a | kcat | kcat/Km | SSb | Km | kcat | kcat/Km | SSb | Km | kcat | kcat/Km | Km | kcat | kcat/Km | ||||||
| P. bharatica CSV86T (A)c | 1827 | 191 | 0.10 | S (1.84) | 1697 | 230 | 0.16 | S (2.1) | 110 | 187 | 1.7 | 366 | 306 | 0.86 | 2 | Homotetramer | 55.8 | 8.25 | Current study |
| P. bharatica CSV86T (B) | 5827 | NAd | NA | M | 9968 | NA | NA | M | 67 | NA | NA | 577 | NA | NA | NA | NA | 51.5 | 6.5 | |
| P. bharatica CSV86T (C) | 1940 | 11.4 | 0.0056 | M | 364 | 11.4 | 0.03 | M | 18 | 8.5 | 0.48 | 3100 | 11.9 | 0.004 | 123 | Homotetramer | 57.5 | 8.25 | |
| T. maritima | 200 | 35000 | 175 | M | NA | NA | NA | NA | 40 | 35000 | 875 | 12000 | 11000 | 0.92 | 955 | Homodimer | 60 | 7.4 | 29 |
| E. coli | NA | NA | NA | NA | NA | NA | NA | NA | 7.5 | 174 | 23.2 | 5090 | 288 | 0.06 | 410 | NA | NA | 8.2 | 18 |
| G. oxydans | 280 | 44 | 0.16 | M | NA | NA | NA | NA | 26 | 43 | 1.6 | 740 | 43 | 0.06 | 28 | Homodimer | 54.5 | 7 | 42 |
| L. mesenteroids | 114 | 523 | 4.6 | M | 69 | 1125 | 16.3 | M | 8 | 522 | 65.3 | 162 | 1125 | 6.9 | 9.4 | Homodimer | - | 7.6 | 39 |
| A. aeolicus 40°C | 15 | NA | NA | M | 58 | NA | NA | M | 9.1 | 47 | 5.1 | 230 | 58 | 0.25 | 20 | Homodimer | 55 | 7 | 41 |
| A. aeolicus 70°C | 63 | NA | NA | M | 180 | NA | NA | M | 161 | 894 | 5.6 | 2096 | 2012 | 0.96 | 5.8 | ||||
| Z. mobilis | NA | NA | NA | M | NA | NA | NA | M | 40 | 338 | 8.5 | 210 | 589 | 2.8 | 3 | Homotetramer | 52 | 8 | 28 |
| P. fluorescens | 2700 | NA | NA | S (1.65) | 2330 | NA | NA | S (1.59) | 360 | 1117 | 3.1 | 150 | 1383 | 9.2 | 0.34 | NA | NA | 8.9 | 15 |
| P. putida KT2440 (A) | 946 | NA | NA | M | 1137 | NA | NA | M | 14 | 102 | 7.3 | 127 | 227 | 1.8 | 4.1 | NA | NA | 8 | 8 |
| P. putida KT2440 (B) | 190 | NA | NA | M | 291 | NA | NA | M | 165 | 113 | 0.68 | 151 | 120 | 0.8 | 0.86 | NA | NA | 8 | 14 |
| P. putida KT2440 (C) | 944 | NA | NA | M | 2030 | NA | NA | M | 3.2 | 0.54 | 0.17 | 9500 | 0.77 | 8.1 × 10−5 | 2082 | NA | NA | 8 | |
| P. aeruginosa (A) | 499 | NA | NA | S (1.99) | 1146 | NA | NA | S (2.38) | 57 | 540 | 9.5 | 527 | 1017 | 1.9 | 4.9 | Homotetramer or octamer | 55 | 8 | 20 |
| A. vinelandii | 530 | NA | NA | S (1.66) | 690 | NA | NA | S (1.76) | 50 | 36.7 | 0.73 | 220 | 91 | 0.41 | 1.8 | Homotetramer | 52 | 8.5 | 16 |
a
Km, μM; kcat, s−1; kcat/Km, μM−1 s−1.
b
SS, substrate saturation profile: S, sigmoidal saturation profile with Hill’s coefficient (n) value in the bracket indicating the allosteric nature of enzyme; M, Michaelis-Menten hyperbolic saturation profile.
c
Zwf A, B, or C isozymes.
d
NA, data not available.