FIG 4.

SRA negatively regulates the TBK1/IRF3 signaling pathway. BMDMs from WT and SRA^−/− mice were infected with VSV (MOI = 0.1) or HSV-1 (MOI = 10). (A, B) The expression of phosphorylated and total TBK1 and IRF3 of BMDMs infected with VSV (A) or HSV-1 (B) at the indicated time points. (C, D) The expression of IRF3 in the cytoplasmic (CE) and nuclear (NE) fractions of BMDMs infected with VSV (C) or HSV-1 (D). ns, not significant; *, P < 0.05; **, P < 0.01; determined by unpaired Student’s t-tests. Data are representative of three independent experiments with similar results.