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. 2022 Nov 7;10(6):e02028-22. doi: 10.1128/spectrum.02028-22

FIG 5.

FIG 5

SRA directly targets TBK1. HEK293T cells were transfected with expression plasmids of Flag-TBK1 or V5-SRA for 48 h. (A, B) The association of V5-SRA with Flag-TBK1 was determined by immunoprecipitation. (C) Representative confocal microscopy photographs of TBK1 (red) and SRA (green) in RAW264.7 cells infected with VSV (MOI = 0.1) or HSV-1 (MOI = 10) for 8 h. (D, E) The association of SRA with TBK1 in macrophages infected with VSV (MOI = 0.1) (D) or HSV-1 (MOI = 10) (E) for 24 h was determined by immunoprecipitation. (F, G) The association of SRA with TBK1 was determined in BMDMs infected with VSV (MOI = 0.1) (F) or HSV-1 (MOI = 10) (G) for 24 h by immunoprecipitation. (H) The association of V5-SRA with truncation mutants of Flag-TBK1 was determined by immunoprecipitation. The top panel shows TBK1 and its truncation mutants. Scale bars = 20 μm. Data are representative of three independent experiments with similar results.