FIG 4.

Dietary supplementation with ALG alters the intestinal BA profile in DSS-treated mice. Mice supplemented with or without 5% ALG for 4 weeks, followed by induction of colitis with 3% DSS in drinking water for another week (n = 8). The colonic digesta was collected from each mouse at the end of the experiment and subjected to targeted BA profiling. (A) PCA plot of the BA profile among three groups of mice. (B) Relative abundances (%) of different BAs among three groups. (C) Z-score plot of all BAs identified among three groups. (D) Alterations of representative BAs among three groups. (E) Spearman correlation between the relative abundance of the intestinal microbiota and BA concentrations. Data are presented as means ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.