FIG 8.

Dietary supplementation with HDCA ameliorates DSS-induced acute colitis. (A) Experimental scheme for DSS-induced colitis and HDCA treatment (n = 12). (B) Body weight was recorded daily in the final week of DSS treatment for three groups of mice. The colon was collected from each mouse at the end of the experiment. (C to F) Representative images of the colon (C), the colon length (D), and H&E staining (magnification, ×50), TEM image of the colon (E), and colonic histology score (F) are shown. (G and H) The mRNA gene expression levels of three inflammatory cytokines (G) and two BA receptors (H) in the colon were also measured by RT-qPCR. Data are presented as the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; #, P < 0.05; ##, P < 0.01; compared with the DSS plus HDCA group.