TABLE 2.

Differences in lipid metabolism protein abundances between VACV-infected and uninfected WT BMDMs

Pathway	Protein ID	Genea	Description	Std log2 (FC)b (VACV versus uninfected Isg15+/+)
Fatty acid oxidation	Q99MN9	Pccb	Propionyl-CoA carboxylase beta chain, mitochondrial	10.02
	P51174	Acadl	Long-chain-specific acyl-CoA dehydrogenase, mitochondrial	8.64
	Q91ZA3	Pcca	Propionyl-CoA carboxylase alpha chain, mitochondrial	8.63
	Q9EPL9	Acox3	Peroxisomal acyl-coenzyme A oxidase 3	8.62
	Q8QZT1	Acat1	Acetyl-CoA acetyltransferase, mitochondrial	7.71
	Q9Z2Z6	Slc25a20	Mitochondrial carnitine/acylcarnitine carrier protein	6.67
	Q9JHI5	Ivd	Isovaleryl-CoA dehydrogenase, mitochondrial	6.39
	Q8BH95	Echs1	Enoyl-CoA hydratase, mitochondrial	5.58
	Q8JZN5	Acad9	Complex I assembly factor ACAD9, mitochondrial	5.39
	Q99JY0	Hadhb	Trifunctional enzyme subunit beta, mitochondrial	5.11
	Q9CQ62	Decr1	2,4-Dienoyl-CoA reductase ([3E]-enoyl-CoA-producing), mitochondrial	4.23
	P50544	Acadvl	Very long-chain-specific acyl-CoA dehydrogenase, mitochondrial	3.55
	Q9DBL1	Acadsb	Short/branched-chain-specific acyl-CoA dehydrogenase, mitochondrial	3.04
	Q60759	Gcdh	Glutaryl-CoA dehydrogenase, mitochondrial	3.02
	Q8K370	Acad10	Acyl-CoA dehydrogenase family member 10	2.12
	Q8CAY6	Acat2	Acetyl-CoA acetyltransferase, cytosolic	−2.61
	P97742	Cpt1a	Carnitine O-palmitoyltransferase 1, liver isoform	−5.88
	Q9CZS1	Aldh1b1	Aldehyde dehydrogenase X, mitochondrial	−6.21
	P32020	Scp2	Sterol carrier protein 2	−6.86
Lipid synthesis	O35083	Agpat1	1-Acyl-sn-glycerol-3-phosphate acyltransferase alpha	4.95
	Q9D517	Agpat3	1-Acyl-sn-glycerol-3-phosphate acyltransferase gamma	4.62
	Q8CHK3	Mboat7	Lysophospholipid acyltransferase 7	4.25
	Q8BHF7	Pgs1	CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase, mitochondrial	4.21
	Q8K3K7	Agpat2	1-Acyl-sn-glycerol-3-phosphate acyltransferase beta	3.29
	Q91YX5	Lpgat1	Acyl-CoA:lysophosphatidylglycerol acyltransferase 1	2.47
	Q8BYI6	Lpcat2	Lysophosphatidylcholine acyltransferase 2	2.44
	Q8JZK9	Hmgcs1	Hydroxymethylglutaryl-CoA synthase C domain-containing protein	−2.34
	Q920E5	Fdps	Farnesyl pyrophosphate synthase	−7.31
	P19096	Fasn	Fatty acid synthase	−8.10
Lipid hydrolysis	Q8VCI0	Plbd1	Phospholipase B-like 1	10.62
	Q99LR1	Abhd12	Lysophosphatidylserine lipase ABHD12	9.89
	Q3TCN2	Plbd2	Putative phospholipase B-like 2	7.85
	Q9Z0M5	Lipa	Lysosomal acid lipase/cholesteryl ester hydrolase	7.12
	Q91WC9	Daglb	Diacylglycerol lipase-beta	5.27
	Q8BLF1	Nceh1	Neutral cholesterol ester hydrolase 1	4.72
	Q9WV54	Asah1	Acid ceramidase	4.12
	Q8VEB4	Pla2g15	Phospholipase A2 group XV	3.61
	Q80YA3	Ddhd1	Phospholipase DDHD1	2.35
	Q8BVA5	Ldah	Lipid droplet-associated hydrolase	−2.57
Lipid transport and storage	P08226	Apoe	Apolipoprotein E	13.09
	Q61263	Soat1	Sterol O-acyltransferase 1	11.56
	Q08857	Cd36	Platelet glycoprotein 4	10.39
	P55302	Lrpap1	Alpha-2-macroglobulin receptor-associated protein	9.58
	Q91ZX7	Lrp1	Prolow-density lipoprotein receptor-related protein 1	7.90
	P41233	Abca1	Phospholipid-transporting ATPase ABCA1	5.22
	P48410	Abcd1	ATP-binding cassette subfamily D member 1	2.79
	P43883	Plin2	Perilipin-2	2.65
	Q61009	Scarb1	Scavenger receptor class B member 1	2.68
	Q00623	Apoa1	Apolipoprotein A-I	2.23
	Q64343	Abcg1	ATP-binding cassette subfamily G member 1	−3.01
	Q3B7Z2	Osbp	Oxysterol-binding protein 1	−4.58
	P11404	Fabp3	Fatty acid-binding protein, heart	−6.93

^aMitochondrial proteins are highlighted in bold, and targets of ISGylation are underlined.

^bFC, fold change; Std, standard.